探讨基质金属蛋白酶-2（matrix metallopro-teinase-2，MMP-2）、基质金属蛋白酶组织抑制因子-2（tissue inhibitor-2，TIMP-2）在不同年龄阶段成人腹股沟疝患者腹直肌前鞘中的表达及意义。

选择2016年2月至2016年11月，郑州大学第一附属医院行手术治疗的58例患者，其中试验组腹股沟疝患者48例，按年龄段分为4组，对照组无疝患者10例，均为男性。取材部位均为下腹部腹直肌前鞘，用实时荧光定量PCR技术检测成人不同年龄阶段组织标本中MMP-2mRNA及TIMP-2 mRNA的表达水平。

MMP-2mRNA表达：对照组1.530±0.612，试验1组1.561±0.222，试验2组2.305±0.213，试验3组2.918±0.392，试验4组3.270±0.259。4个试验组与对照组比较，均呈高表达，试验3组及4组分别与对照组比较，差异均有统计学意义（P<0.05），试验1组及2组分别与对照组比较，差异均无统计学意义（P>0.05）。4个试验组相互比较，随着年龄增大表达逐渐增高，1组与3组比较、1组与4组比较、2组与4组比较差异均有统计学意义（P<0.05），其余组间比较差异均无统计学意义（P>0.05）。TIMP-2 mRNA表达：对照组1.021±0.395，试验1组0.996±0.096，试验2组0.813±0.049，试验3组0.504±0.035，试验4组0.374±0.026。4个试验组与对照组比较，均呈低表达，试验3组及4组分别与对照组比较差异均有统计学意义（P<0.05），试验1组及2组分别与对照组比较差异均无统计学意义（P>0.05）。4个试验组相互比较，随着年龄增大表达逐渐降低，1组与3组比较、1组与4组比较、2组与4组比较差异均有统计学意义（P<0.05），其余组间比较差异均无统计学意义（P>0.05）。4个试验组均值：MMP-2 mRNA为2.513±1.135、TIMP-2 mRNA为0.672±0.313，与对照组1.530±0.612、1.021±0.395比较，差异均有统计学意义（P<0.05）。

成人腹股沟疝患者腹直肌前鞘中，MMP-2mRNA呈高表达，TIMP-2mRNA呈低表达，与无疝者比较有显著差异，表明2者与腹股沟疝的发生密切相关。随着年龄增大，MMP-2mRNA逐渐增高，TIMP-2mRNA逐渐降低，70岁以上表达显著，这是老年人随着年龄增大腹股沟疝患病率增高的重要原因之一。

To investigate the association of MMP-2 and TIMP -2 in adults' anterior rectus sheath of patients with inguinal hernia in different age stage.

There were fifty-eight patients undergoing surgical treatment in the First Affiliated Hospital of Zhengzhou University from February 2016 to November 2016, including forty-eight cases of inguinal hernia in the experimental group and ten cases of non-hernia in the control group, all of whom male. The expression levels of MMP-2mRNA and TIMP-2mRNA of the cases at different age were detected by Real-Time PCR.

The expression of MMP-2mRNA: the control group was 1.530±0.612, the group 1 was 1.561±0.222, the group 2 was 2.305±0.213, the group 3 was 2.918±0.392, the group 4 was 3.270±0.259. The four experimental groups showed high expression compared with the control group. The differences were significant comparing the group 3 and the group 4 with the control one, and there was no statistical significance to compare the group 1 and the group 2 with the control one. When compared with each other between the 4 groups, the expression gradually increased with age. The expression of TIMP-2mRNA: the control group was 1.021±0.395, the group 1 was 0.996±0.096, the group 2 was 0.813±0.049, the group 3 was 0.504±0.035, the group 4 was 0.374±0.026. The four experimental groups showed low expression comparing with the control group. The differences were significant comparing the group 3 and the group 4 with the control one, and there was no statistical significance to compare the group 1 and the group 2 with the control one. When compared with each other between the 4 groups, the expression gradually decreased with age. The average value of MMP-2mRNA and TIMP-2mRNA of 4 groups were significantly different with the control group, respectively.

The expression of MMP-2mRNA in the anterior sheath of the abdominal rectus abdominis in adult inguinal hernia was high, and the low expression of TIMP-2mRNA was significantly different from that of the non-hernia, indicating that the two were closely related to the occurrence of inguinal hernia. As the age increase, MMP-2mRNA gradually increase, and TIMP-2mRNA gradually decreases. This is one of the important reasons for the increase of the prevalence of inguinal hernia with age.