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中华疝和腹壁外科杂志(电子版)

中华疝和腹壁外科杂志(电子版) ›› 2024, Vol. 18 ›› Issue (02) : 161 -167. doi: 10.3877/cma.j.issn.1674-392X.2024.02.007

疝修补材料专栏

不同材质补片修补对腹壁疝大鼠腹横筋膜组织转化生长因子-β1及Collagen合成代谢的作用
赛甫丁·艾比布拉1, 买买提·依斯热依力2, 李义亮1, 王永康3, 王志4, 克力木·阿不都热依木2,†()   
  1. 1. 830001　乌鲁木齐，新疆维吾尔自治区人民医院微创、疝和腹壁外科；830001　乌鲁木齐，新疆维吾尔自治区人民医院新疆胃食管反流病与减重代谢外科临床医学研究中心
    2. 830001　乌鲁木齐，新疆维吾尔自治区人民医院微创、疝和腹壁外科；830001　乌鲁木齐，新疆维吾尔自治区人民医院新疆胃食管反流病与减重代谢外科临床医学研究中心；830001　乌鲁木齐，新疆维吾尔自治区人民医院自治区普外微创研究所
    3. 830001　乌鲁木齐，新疆维吾尔自治区人民医院自治区普外微创研究所
    4. 830001　乌鲁木齐，新疆维吾尔自治区人民医院微创、疝和腹壁外科
  • 收稿日期:2022-10-19 出版日期:2024-04-18
  • 通信作者: 克力木·阿不都热依木
  • 基金资助:
    新疆维吾尔自治区自然科学基金(2022D01C108，2020D01C120)

Role of different mesh materials on transforming growth factor-beta1 and Collagen anabolism on the abdominal transverse fascia of rat with abdominal wall hernia

Aibibula Saifuding·1, Yisireyili Maimaiti·2, Yiliang Li1, Yongkang Wang3, Zhi Wang4, Abudureyimu Kelimu·2,†()   

  1. 1. Department of Minimally Invasive Surgery, Hernia and Abdominal Wall Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China; Xinjiang Clinical Research Center for Gastroesophageal Reflux Disease and Weight Loss Metabolic Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
    2. Department of Minimally Invasive Surgery, Hernia and Abdominal Wall Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China; Xinjiang Clinical Research Center for Gastroesophageal Reflux Disease and Weight Loss Metabolic Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China; Research Institute of General and Minimally Invasive Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
    3. Research Institute of General and Minimally Invasive Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
    4. Department of Minimally Invasive Surgery, Hernia and Abdominal Wall Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
  • Received:2022-10-19 Published:2024-04-18
  • Corresponding author: Abudureyimu Kelimu·
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赛甫丁·艾比布拉, 买买提·依斯热依力, 李义亮, 王永康, 王志, 克力木·阿不都热依木. 不同材质补片修补对腹壁疝大鼠腹横筋膜组织转化生长因子-β1及Collagen合成代谢的作用[J]. 中华疝和腹壁外科杂志(电子版), 2024, 18(02): 161-167.

Aibibula Saifuding·, Yisireyili Maimaiti·, Yiliang Li, Yongkang Wang, Zhi Wang, Abudureyimu Kelimu·. Role of different mesh materials on transforming growth factor-beta1 and Collagen anabolism on the abdominal transverse fascia of rat with abdominal wall hernia[J]. Chinese Journal of Hernia and Abdominal Wall Surgery(Electronic Edition), 2024, 18(02): 161-167.

目的

探讨两种不同材质（聚丙烯、复合）补片修补对腹壁疝大鼠腹横筋膜组织转化生长因子-β1（TGF-β1）及Collagen合成代谢的影响及作用。

方法

选取8周龄，SPF级雄性SD大鼠随机分为4组，即正常对照组、腹壁疝组、聚丙烯补片修补组、复合补片修补组，每组6只。大鼠下腹部以钝性分离皮肤与肌肉组织后切除3 cm×2 cm大小腹壁肌肉筋膜组织来造腹壁缺损（AH）模型，并采用聚丙烯补片（PM）、复合补片（CM）材质补片进行肌筋膜前置（Onlay）方式修补；4组大鼠术后均饲养8周后处死，取出腹壁全层（补片及腹横筋膜）组织，加入TRIzol裂解液，取总RNA；采用RT-PCR实验方法检测TGF-β1、Smad2/3、胶原蛋白（Collagen Ⅰ型和Ⅲ型）及基质金属蛋白酶（MMP-2、MMP-9）的mRNA表达水平。

结果

AH大鼠显著降低腹横筋膜组织TGF-β1、Smad-2以及Smad-3的mRNA表达水平，与正常对照组相比差异有统计学意义（P<0.001）；PM及CM修补均有效的增加TGF-β1、Smad-2以及Smad-3的mRNA水平（P<0.01）；而复合补片组明显增高mRNA表达水平，与聚丙烯补片组相比，差异有统计学意义（P<0.05）。AH大鼠显著降低腹横筋膜组织中Collagen Ⅰ和Collagen Ⅲ的mRNA表达水平（P<0.001）；复合补片组更有效的增高Collagen（Ⅰ型和Ⅲ型）的mRNA表达水平，与聚丙烯补片组相比，差异有统计学意义（P<0.05）。AH大鼠显著增高腹横筋膜组织中MMP-2和MMP-9的mRNA表达水平（P<0.001）；复合补片组更有效的降低MMP-2和MMP-9的mRNA表达水平，与聚丙烯补片组相比，差异有统计学意义（P<0.05）。

结论

聚丙烯补片和复合补片修补均可以改善AH大鼠腹横筋膜组织TGF-β1及Collagen的合成，而复合补片更加有效的促进胶原蛋白的合成。

关键词: 腹壁疝, 大鼠，模型, 聚丙烯补片, 复合补片, 转化生长因子β1, 胶原蛋白
Objective

To explore the effects of two different materials (polypropylene, composite) meshes on transforming growth factor-beta1 (TGF-β1) and Collagen anabolism of abdominal transverse fascia in rats with abdominal wall hernia.

Methods

Eight-week-old male SPF grade SD rats were randomly divided into four groups: control group (Control), abdominal wall hernia (AH), polypropylene mesh repair (PM) and composite mesh repair (CM) group, with 6 rats in each group. The lower abdomen of rats was cut after blunt separation of skin and muscle tissue. The model of abdominal wall defect was made by removing 3 cm×2 cm full thickness abdominal wall tissue, and was repaired with PM and CM mesh materials using Onlay mode respectively. The rats in the four groups were killed after 8 weeks of feeding. The whole layer of abdominal wall (mesh and transverse fascia) was taken out, added to TRIzol lysate buffer, and total RNA was taken; the RT-PCR method was used to detect the mRNA expression levels of TGF-β1, Smad-2, Smad-3, CollagenⅠand Ⅲ, and matrix metalloproteinase (MMP-2, MMP-9).

Results

Compared to control group, the mRNA expression levels of TGF-β1, Smad-2 and Smad-3 in transverse abdominal fascia of AH rats were significantly decreased (P<0.001); Both PM and CM groups can effectively increase the levels of TGF-β1 and Smad-2/3 mRNA expressions (P<0.01), and CM group had significantly higher mRNA expression than PM group (P<0.05). AH rats significantly decreased the mRNA expression of CollagenⅠand Collagen Ⅲ in the abdominal transverse fascia (P<0.001); The CM group was more effective in increasing the mRNA expression level of Collagen (typeⅠand Ⅲ) than the PM group, and the difference was significant (P<0.05). AH rats significantly increased the mRNA expression levels of MMP-2 and MMP-9 in the abdominal transverse fascia (P<0.001); The mRNA expression levels of MMP-2 and MMP-9 in the CM group were significantly lower than those in the PM group (P<0.05).

Conclusion

The polypropylene mesh and composite mesh could improve the TGF-β1 and Collagen anabolism of transverse abdominal fascia in AH rats, and the composite mesh was more effectively in promoting the synthesis of Collagen.

Key words: Abdominal wall hernia, Rat, model, Polypropylene mesh, Composite mesh, Transforming growth factor β1, Collagen
图1 聚丙烯补片修补大鼠腹壁缺损
图2 复合补片修补大鼠腹壁缺损
表1 实时定量RT-PCR引物序列
指标 上游引物(5'-3') 下游引物(5'-3')
TGFβ-1 TGCACATCCGTGCAGAGAAT CTGGGTTCTGCTTGTTTCGC
Smad-2 GCCGCCCGAAGGGTAGAT GAGGCAAGACTGGTGTCTCC
Smad-3 TTAAAAGCGAAGTTCGGGCG TGTGGTTCATCTGGTGGTCG
MMP-2 GATCCCCAGAGCGTTACTCG GTTGTGGAAACTCACACGCC
MMP-9 CTGACGGGCCGTACAATCTT CCACCTTGGCACACCTGTAT
CollagenⅠ GCTGGGATGACTGCTACTGG AAGCGGGGTTAATGGGCAAT
Collagen Ⅲ TCTGGCGGCTTTTCACCATA GCAATTGCGAGCAGGGTTTT
GAPDH GCATCTTCTTGTGCAGTGCC GATGGTGATGGGTTTCCCGT
图3 4组大鼠腹横筋膜组织TGF-β1和Smad-2/3的mRNA表达情况注：数据以±s表示，n=6；与control组比较，***P<0.001；与AH组比较，##P<0.01；与PM组比较，△P<0.05。Control为正常对照组，AH为腹壁疝组，PM为聚丙烯补片修补组，CM为复合补片修补组。
图4 4组大鼠腹横筋膜组织胶原蛋白的mRNA表达情况注：数据以±s表示，n=6；与control组比较，***P<0.001；与AH组比较，##P<0.01；与PM组比较，△P<0.05。Control为正常对照组，AH为腹壁疝组，PM为聚丙烯补片修补组，CM为复合补片修补组。
图5 4组大鼠腹横筋膜组织基质金属蛋白酶的mRNA表达情况注：数据以±s表示，n=6；与control组比较，***P<0.001；与AH组比较，##P<0.01；与PM组比较，△P<0.05。Control为正常对照组，AH为腹壁疝组，PM为聚丙烯补片修补组，CM为复合补片修补组。
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