To explore objective and accurate pathological detection indexes and evaluation methods based on the requirements of "Guidelines for technical review of intraperitoneal hernia repair mesh in animal experiments" .

Two kinds of composite hernia patches were implanted in the abdominal wall of dogs. After 12 weeks, the patch area and surrounding normal tissues were sampled, and the proportion of peritoneal mesothelial cells, the inflammatory response, and neovascularization were evaluated by immunohistochemistry and immunofluorescence staining. The degree of fibrosis was detected by Masson staining. Fat infiltration was detected by HE staining.

(1) Both Cytokeratin and Vimentin could accurately identify mesothelial cells, which is helpful to measure the length of mesothelial cell layer; (2) Using antibodies CD20, CD3 and CD68 could effectively detect B lymphocytes, T lymphocytes and macrophages in tissues; (3) Using CD34 antibody could be used to observe the situation and calculate the density of neovascularization. (4) HE staining could be used for observe the infiltration of adipocytes and semi-quantitative analysis; (5) Masson staining could clearly reflect the deposition of collagen fiber, and the thickness of the fiber deposition area was measured and compared; (6) All observation indicators of the two groups were compared by quantitative and semi-quantitative methods, and there was no significant difference (P>0.05).

The detection method adopted in this study could directly and accurately locate and quantify the indicators of local response, which basically met the requirements of "Guidelines for technical review of intraperitoneal hernia repair mesh in animal experiments" , and has a certain reference value for future relevant experiments.