Abstract:

Objective

To detect the changes of vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) in serum at different times after preperitoneal space mesh repair for incisional hernia in rats, and explore the fusion rate of mesh and tissue.

Methods

A total of 120 SD rats were randomly divided into control group (n=12), incisional hernia model group (n=12), hernia-free mesh group 1, 4, 8, 12 weeks after surgery (n=12 in each group), and 1, 4, 8 and 12 weeks after hernia mesh repair(n=12 in each group).Implant polypropylene mesh into the preperitoneal space of rats in the hernia-free implantation mesh group and the hernia mesh repair group.The control group took the blood of normal rats, the incision hernia model group took the blood 2 weeks after modeling, and the blood of 1, 4, 8 and 12 weeks after the implantation mesh was taken from the hernia implant-free mesh group and the hernia mesh repair group, and the VEGF and Ang-1 contents in serum were measured.

Results

Compared with the control group, the serum concentrations of VEGF and Ang-1 in the incisional hernia model group increased significantly (P＜0.05), the concentrations of VEGF and Ang-1 increased (P＜0.05) in the group without hernia implantation mesh group 1 week after surgery, and there was no significant difference in VEGF concentration in the 4, 8 and 12 weeks after surgery (P＞0.05), while the concentration of Ang-1 increased in the 4 and 8 weeks postoperative group (P＜0.05), and there was no significant difference in the group 12 weeks after surgery (P＞0.05); Compared with the incision hernia model group, there was no significant difference in VEGF and Ang-1 concentrations in the hernia mesh repair group at 1, 4 and 8 weeks after surgery (P＞0.05), and the VEGF concentration decreased (P＜0.05) and Ang-1 concentration (P＞0.05) in the hernia mesh repair group.Compared with the hernia implantation mesh group, the level of Ang-1 increased (P＜0.05) in the hernia mesh repair group 12 weeks after surgery, and there was no significant difference in VEGF and Ang-1 concentrations in the remaining periods (P＞0.05).

Conclusion

After preperitoneal space mesh repair for extra-abdominal hernia in rats, surgical trauma caused a significant increase in VEGF and Ang-1 in serum, while stimulation of the mesh foreign body did not cause an additional significant increase in VEGF and Ang-1 in serum, indicating that the fusion of mesh and tissue may be a slow process, suggesting that strenuous activities should be avoided in the short term after surgery, resulting in the displacement of mesh that has not been well integrated with tissue.

Key words: Abdominal external hernia, Preperitoneal space, Mesh, Hernia repair, Vascular endothelial growth factor, Angiotensin-1