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中华疝和腹壁外科杂志(电子版)

中华疝和腹壁外科杂志(电子版) ›› 2023, Vol. 17 ›› Issue (02) : 125 -129. doi: 10.3877/cma.j.issn.1674-392X.2023.02.002

基础论著

聚丙烯和生物补片对腹壁疝大鼠腹横筋膜组织氧化应激、MMPs及TIMPs的影响
李义亮1, 买买提·依斯热依力2, 王永康3, 王志4, 赛甫丁·艾比布拉4, 李赞林4, 克力木·阿不都热依木1,†()   
  1. 1. 830001　乌鲁木齐，新疆维吾尔自治区人民医院微创、疝和腹壁外科；830001　乌鲁木齐，新疆维吾尔自治区胃食管反流病及减重代谢外科临床研究中心；830001　乌鲁木齐，新疆维吾尔自治区人民医院自治区普外微创研究所
    2. 830001　乌鲁木齐，新疆维吾尔自治区人民医院微创、疝和腹壁外科；830001　乌鲁木齐，新疆维吾尔自治区胃食管反流病及减重代谢外科临床研究中心；830001　乌鲁木齐，新疆维吾尔自治区人民医院自治区普外微创研究所；830001　乌鲁木齐，新疆维吾尔自治区人民医院医学研究与转化中心
    3. 830001　乌鲁木齐，新疆维吾尔自治区人民医院微创、疝和腹壁外科；830001　乌鲁木齐，新疆维吾尔自治区人民医院自治区普外微创研究所
    4. 830001　乌鲁木齐，新疆维吾尔自治区人民医院微创、疝和腹壁外科；830001　乌鲁木齐，新疆维吾尔自治区胃食管反流病及减重代谢外科临床研究中心
  • 收稿日期:2022-10-01 出版日期:2023-04-18
  • 通信作者: 克力木·阿不都热依木
  • 基金资助:
    新疆维吾尔自治区自然科学基金(2020D01C120，2022D01C108)

Effects of polypropylene and biological mesh on oxidative stress, MMPs and TIMPs in abdominal transverse fascia of rats with abdominal hernia

Yiliang Li1, Yisireyili Maimaiti2, Yongkang Wang3, Zhi Wang4, Aibibula Saifuding4, Zanlin Li4, Abudureyimu Kelimu1,†()   

  1. 1. Department of Minimally Invasive Surgery, Hernia and Abdominal Wall Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830001, China; Xinjiang Clinical Research Center for Gastroesophageal Reflux Disease and Weight Loss Metabolic Surgery, Urumqi, 830001, China; Research Institute of General and Minimally Invasive Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830001, China
    2. Department of Minimally Invasive Surgery, Hernia and Abdominal Wall Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830001, China; Xinjiang Clinical Research Center for Gastroesophageal Reflux Disease and Weight Loss Metabolic Surgery, Urumqi, 830001, China; Research Institute of General and Minimally Invasive Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830001, China; Medical Research and Transformation Center, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830001, China
    3. Department of Minimally Invasive Surgery, Hernia and Abdominal Wall Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830001, China; Research Institute of General and Minimally Invasive Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830001, China
    4. Department of Minimally Invasive Surgery, Hernia and Abdominal Wall Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830001, China; Xinjiang Clinical Research Center for Gastroesophageal Reflux Disease and Weight Loss Metabolic Surgery, Urumqi, 830001, China
  • Received:2022-10-01 Published:2023-04-18
  • Corresponding author: Abudureyimu Kelimu
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引用本文：

李义亮, 买买提·依斯热依力, 王永康, 王志, 赛甫丁·艾比布拉, 李赞林, 克力木·阿不都热依木. 聚丙烯和生物补片对腹壁疝大鼠腹横筋膜组织氧化应激、MMPs及TIMPs的影响[J/OL]. 中华疝和腹壁外科杂志(电子版), 2023, 17(02): 125-129.

Yiliang Li, Yisireyili Maimaiti, Yongkang Wang, Zhi Wang, Aibibula Saifuding, Zanlin Li, Abudureyimu Kelimu. Effects of polypropylene and biological mesh on oxidative stress, MMPs and TIMPs in abdominal transverse fascia of rats with abdominal hernia[J/OL]. Chinese Journal of Hernia and Abdominal Wall Surgery(Electronic Edition), 2023, 17(02): 125-129.

目的

探讨聚丙烯补片及生物补片修补对腹壁疝大鼠腹横筋膜组织中氧化应激、基质金属蛋白酶（MMPs）及其抑制剂（TIMPs）表达的作用。

方法

8周龄，SPF级雄性SD大鼠随机分为4组，即Control组（正常对照，n=6）、AH组（腹壁疝，n=6）、PM组（聚丙烯补片修补，n=6），BM组（生物补片修补，n=6）。大鼠下腹部以钝性分离皮肤与肌肉组织后切除3 cm×2 cm腹壁肌肉筋膜组织来造腹壁缺损（AH）模型，并采用不同材质（PM、BM）补片进行肌筋膜前置方式修补；术后4组大鼠均饲养8周后处死，采取血液标本并取出补片以及腹横筋膜组织；采用ELISA和RT-PCR等方法检测MMPs（MMP-2、MMP-9）、TIMPs（TIMP-1、TIMP-2）、氧化应激（MDA）及抗氧化酶（Mn-SOD、GSH-Px、CAT）的表达水平。

结果

与Control组相比，血清中MDA的水平AH组大鼠显著增高（P<0.01）；PM组及BM组较AH组其水平均有效降低（P<0.01）；且BM组降低效果比PM组显著（P<0.05）。腹横筋膜组织中抗氧化酶（Mn-SOD、GSH-Px、CAT）的mRNA表达水平AH组大鼠显著降低（P<0.001）；PM组及BM组较AH组均升高（P<0.01），且BM组比PM组升高显著（P<0.05）。与Control组相比，AH组大鼠腹横筋膜组织中MMP-2、MMP-9的mRNA表达水平显著增高（P<0.01），同时其TIMP-1、TIMP-2的mRNA表达水平降低（P<0.001）；PM组及BM组较AH组大鼠MMP-2、MMP-9、TIMP-1、TIMP-2的异常表达均得到改善（P<0.01），且BM组优于PM组。

结论

聚丙烯补片及生物补片均可改善氧化应激、MMPs及TIMPs的表达水平，而生物补片优于聚丙烯补片，更深入的机制有待进一步探索。

关键词: 腹壁疝, 大鼠，模型, 聚丙烯补片, 生物补片, 基质金属蛋白酶
Objective

To explore the effect of polypropylene mesh and biological mesh repair on oxidative stress, matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in the transverse fascia tissue of rats with abdominal wall hernia.

Methods

Eight week old SPF male SD rats were randomly divided into four groups: control group (Control, n=6), abdominal wall hernia group (AH, n=6), polypropylene mesh repair group (PM, n=6), and biological mesh repair group (BM, n=6). The lower abdomen of rats was cut after blunt separation of skin and muscle tissue.The model of an abdominal wall defect (AH) was made with 3 cm×2 cm musculofascial tissue of the abdominal wall, and was repaired with different materials (PM, BM) by using Onlay repairing mode; The rats in the four groups were fed for 8 weeks after operation. The rats in the four groups were killed after 8 weeks of feeding. Blood samples, meshes and transversalis fascia tissue were taken; The expression levels of MMPs (MMP-2, MMP-9), TIMPs (TIMP-1, TIMP-2), oxidative stress (MDA) and antioxidant enzymes (Mn SOD, GSH-Px, CAT) were detected by ELISA and RT-PCR.

Results

Compared with the control group, the level of MDA in serum of AH rats was significantly higher (P<0.01); PM and BM repair can effectively reduce their levels (P<0.01); More importantly, the level of MDA in the BM group was significantly lower than that in the PM group (P<0.05). Compared with the control group, AH rats significantly decreased the mRNA expression of antioxidant enzymes (Mn-SOD, GSH-Px, CAT) in the abdominal transverse fascia (P<0.001); PM and BM could increase the mRNA expression of antioxidant enzymes (P<0.01). More importantly, the level of antioxidant enzymes in the BM group was significantly higher than that in the PM group (P<0.05). Compared with the control group, the mRNA levels of MMP-2 and MMP-9 in the abdominal transverse fascia of AH rats were significantly increased (P<0.01); same time, it decreased the mRNA expression of TIMP-1 and TIMP-2 (P<0.001); Polypropylene mesh(PM) and biological mesh (BM) improved the abnormal expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 in AH rats (P<0.01), and BM group was better than PM group.

Conclusion

Both polypropylene mesh and biological mesh can improve the expression level of oxidative stress, MMPs and TIMPs, but biological mesh is better than polypropylene mesh, so the deeper mechanism needs to be further explored.

Key words: Abdominal wall hernia, Rat, model, Polypropylene mesh, Biological mesh, Matrix metalloproteinase
图1 聚丙烯补片修补大鼠腹壁疝实验
图2 生物补片修补大鼠腹壁疝实验
表1 实时定量RT-PCR引物序列
指标 上游引物(5'-3') 下游引物(5'-3')
MMP-2 GATCCCCAGAGCGTTACTCG GTTGTGGAAACTCACACGCC
MMP-9 CTGACGGGCCGTACAATCTT CCACCTTGGCACACCTGTAT
TIMP-1 GGCATAATCTGAGCCCTGCT TTGCAAGGGATGGCTGAACA
TIMP-2 GATCACACGCTGCCCTATGA AGCGTGAACCCACTTGGATG
MnSOD CCCGCGAGTACAACCTTCTT AACACAGCCTGGATGGCTAC
GSH-Px TGCGTCTGGAAGTAAAGCGT TATCTCGCGGTTGTCGTAGC
CAT GCTCCGCAATCCTACACCAT GTGGTCAGGACATCGGGTTT
β-actin TAAAACAACCCTTTGGCGGC AGCCACACAGGAATCGGAAG
图3 各组大鼠腹横筋膜组织氧化应激和抗氧化酶的表达水平注：每组n=6；数据均以±s表示；与Control组比较，**P<0.01，***P<0.001；与AH组比较，#P<0.05，##P<0.01；与PM组比较，*P<0.05。
图4 各组大鼠腹横筋膜组织MMPs及TIMPs的mRNA表达水平注：每组n=6；数据均以±s表示；与Control组比较，**P<0.01，***P<0.001；与AH组比较，#P<0.05，##P<0.01；与PM组比较，*P<0.05。
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