切换至 "中华医学电子期刊资源库"
高级检索
中华疝和腹壁外科杂志(电子版)

中华疝和腹壁外科杂志(电子版) ›› 2023, Vol. 17 ›› Issue (02) : 125 -129. doi: 10.3877/cma.j.issn.1674-392X.2023.02.002

基础论著

聚丙烯和生物补片对腹壁疝大鼠腹横筋膜组织氧化应激、MMPs及TIMPs的影响
李义亮1, 买买提·依斯热依力2, 王永康3, 王志4, 赛甫丁·艾比布拉4, 李赞林4, 克力木·阿不都热依木1,†()   
  1. 1. 830001　乌鲁木齐，新疆维吾尔自治区人民医院微创、疝和腹壁外科；830001　乌鲁木齐，新疆维吾尔自治区胃食管反流病及减重代谢外科临床研究中心；830001　乌鲁木齐，新疆维吾尔自治区人民医院自治区普外微创研究所
    2. 830001　乌鲁木齐，新疆维吾尔自治区人民医院微创、疝和腹壁外科；830001　乌鲁木齐，新疆维吾尔自治区胃食管反流病及减重代谢外科临床研究中心；830001　乌鲁木齐，新疆维吾尔自治区人民医院自治区普外微创研究所；830001　乌鲁木齐，新疆维吾尔自治区人民医院医学研究与转化中心
    3. 830001　乌鲁木齐，新疆维吾尔自治区人民医院微创、疝和腹壁外科；830001　乌鲁木齐，新疆维吾尔自治区人民医院自治区普外微创研究所
    4. 830001　乌鲁木齐，新疆维吾尔自治区人民医院微创、疝和腹壁外科；830001　乌鲁木齐，新疆维吾尔自治区胃食管反流病及减重代谢外科临床研究中心
  • 收稿日期:2022-10-01 出版日期:2023-04-18
  • 通信作者: 克力木·阿不都热依木
  • 基金资助:
    新疆维吾尔自治区自然科学基金(2020D01C120，2022D01C108)

Effects of polypropylene and biological mesh on oxidative stress, MMPs and TIMPs in abdominal transverse fascia of rats with abdominal hernia

Yiliang Li1, Yisireyili Maimaiti2, Yongkang Wang3, Zhi Wang4, Aibibula Saifuding4, Zanlin Li4, Abudureyimu Kelimu1,†()   

  1. 1. Department of Minimally Invasive Surgery, Hernia and Abdominal Wall Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830001, China; Xinjiang Clinical Research Center for Gastroesophageal Reflux Disease and Weight Loss Metabolic Surgery, Urumqi, 830001, China; Research Institute of General and Minimally Invasive Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830001, China
    2. Department of Minimally Invasive Surgery, Hernia and Abdominal Wall Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830001, China; Xinjiang Clinical Research Center for Gastroesophageal Reflux Disease and Weight Loss Metabolic Surgery, Urumqi, 830001, China; Research Institute of General and Minimally Invasive Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830001, China; Medical Research and Transformation Center, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830001, China
    3. Department of Minimally Invasive Surgery, Hernia and Abdominal Wall Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830001, China; Research Institute of General and Minimally Invasive Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830001, China
    4. Department of Minimally Invasive Surgery, Hernia and Abdominal Wall Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830001, China; Xinjiang Clinical Research Center for Gastroesophageal Reflux Disease and Weight Loss Metabolic Surgery, Urumqi, 830001, China
  • Received:2022-10-01 Published:2023-04-18
  • Corresponding author: Abudureyimu Kelimu
全文 ( ) 下载PDF ( ) 导出引用
引用本文：

李义亮, 买买提·依斯热依力, 王永康, 王志, 赛甫丁·艾比布拉, 李赞林, 克力木·阿不都热依木. 聚丙烯和生物补片对腹壁疝大鼠腹横筋膜组织氧化应激、MMPs及TIMPs的影响[J]. 中华疝和腹壁外科杂志(电子版), 2023, 17(02): 125-129.

Yiliang Li, Yisireyili Maimaiti, Yongkang Wang, Zhi Wang, Aibibula Saifuding, Zanlin Li, Abudureyimu Kelimu. Effects of polypropylene and biological mesh on oxidative stress, MMPs and TIMPs in abdominal transverse fascia of rats with abdominal hernia[J]. Chinese Journal of Hernia and Abdominal Wall Surgery(Electronic Edition), 2023, 17(02): 125-129.

目的

探讨聚丙烯补片及生物补片修补对腹壁疝大鼠腹横筋膜组织中氧化应激、基质金属蛋白酶（MMPs）及其抑制剂（TIMPs）表达的作用。

方法

8周龄，SPF级雄性SD大鼠随机分为4组，即Control组（正常对照，n=6）、AH组（腹壁疝，n=6）、PM组（聚丙烯补片修补，n=6），BM组（生物补片修补，n=6）。大鼠下腹部以钝性分离皮肤与肌肉组织后切除3 cm×2 cm腹壁肌肉筋膜组织来造腹壁缺损（AH）模型，并采用不同材质（PM、BM）补片进行肌筋膜前置方式修补；术后4组大鼠均饲养8周后处死，采取血液标本并取出补片以及腹横筋膜组织；采用ELISA和RT-PCR等方法检测MMPs（MMP-2、MMP-9）、TIMPs（TIMP-1、TIMP-2）、氧化应激（MDA）及抗氧化酶（Mn-SOD、GSH-Px、CAT）的表达水平。

结果

与Control组相比，血清中MDA的水平AH组大鼠显著增高（P<0.01）；PM组及BM组较AH组其水平均有效降低（P<0.01）；且BM组降低效果比PM组显著（P<0.05）。腹横筋膜组织中抗氧化酶（Mn-SOD、GSH-Px、CAT）的mRNA表达水平AH组大鼠显著降低（P<0.001）；PM组及BM组较AH组均升高（P<0.01），且BM组比PM组升高显著（P<0.05）。与Control组相比，AH组大鼠腹横筋膜组织中MMP-2、MMP-9的mRNA表达水平显著增高（P<0.01），同时其TIMP-1、TIMP-2的mRNA表达水平降低（P<0.001）；PM组及BM组较AH组大鼠MMP-2、MMP-9、TIMP-1、TIMP-2的异常表达均得到改善（P<0.01），且BM组优于PM组。

结论

聚丙烯补片及生物补片均可改善氧化应激、MMPs及TIMPs的表达水平，而生物补片优于聚丙烯补片，更深入的机制有待进一步探索。

关键词: 腹壁疝, 大鼠，模型, 聚丙烯补片, 生物补片, 基质金属蛋白酶
Objective

To explore the effect of polypropylene mesh and biological mesh repair on oxidative stress, matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in the transverse fascia tissue of rats with abdominal wall hernia.

Methods

Eight week old SPF male SD rats were randomly divided into four groups: control group (Control, n=6), abdominal wall hernia group (AH, n=6), polypropylene mesh repair group (PM, n=6), and biological mesh repair group (BM, n=6). The lower abdomen of rats was cut after blunt separation of skin and muscle tissue.The model of an abdominal wall defect (AH) was made with 3 cm×2 cm musculofascial tissue of the abdominal wall, and was repaired with different materials (PM, BM) by using Onlay repairing mode; The rats in the four groups were fed for 8 weeks after operation. The rats in the four groups were killed after 8 weeks of feeding. Blood samples, meshes and transversalis fascia tissue were taken; The expression levels of MMPs (MMP-2, MMP-9), TIMPs (TIMP-1, TIMP-2), oxidative stress (MDA) and antioxidant enzymes (Mn SOD, GSH-Px, CAT) were detected by ELISA and RT-PCR.

Results

Compared with the control group, the level of MDA in serum of AH rats was significantly higher (P<0.01); PM and BM repair can effectively reduce their levels (P<0.01); More importantly, the level of MDA in the BM group was significantly lower than that in the PM group (P<0.05). Compared with the control group, AH rats significantly decreased the mRNA expression of antioxidant enzymes (Mn-SOD, GSH-Px, CAT) in the abdominal transverse fascia (P<0.001); PM and BM could increase the mRNA expression of antioxidant enzymes (P<0.01). More importantly, the level of antioxidant enzymes in the BM group was significantly higher than that in the PM group (P<0.05). Compared with the control group, the mRNA levels of MMP-2 and MMP-9 in the abdominal transverse fascia of AH rats were significantly increased (P<0.01); same time, it decreased the mRNA expression of TIMP-1 and TIMP-2 (P<0.001); Polypropylene mesh(PM) and biological mesh (BM) improved the abnormal expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 in AH rats (P<0.01), and BM group was better than PM group.

Conclusion

Both polypropylene mesh and biological mesh can improve the expression level of oxidative stress, MMPs and TIMPs, but biological mesh is better than polypropylene mesh, so the deeper mechanism needs to be further explored.

Key words: Abdominal wall hernia, Rat, model, Polypropylene mesh, Biological mesh, Matrix metalloproteinase
图1 聚丙烯补片修补大鼠腹壁疝实验
图2 生物补片修补大鼠腹壁疝实验
表1 实时定量RT-PCR引物序列
指标 上游引物(5'-3') 下游引物(5'-3')
MMP-2 GATCCCCAGAGCGTTACTCG GTTGTGGAAACTCACACGCC
MMP-9 CTGACGGGCCGTACAATCTT CCACCTTGGCACACCTGTAT
TIMP-1 GGCATAATCTGAGCCCTGCT TTGCAAGGGATGGCTGAACA
TIMP-2 GATCACACGCTGCCCTATGA AGCGTGAACCCACTTGGATG
MnSOD CCCGCGAGTACAACCTTCTT AACACAGCCTGGATGGCTAC
GSH-Px TGCGTCTGGAAGTAAAGCGT TATCTCGCGGTTGTCGTAGC
CAT GCTCCGCAATCCTACACCAT GTGGTCAGGACATCGGGTTT
β-actin TAAAACAACCCTTTGGCGGC AGCCACACAGGAATCGGAAG
图3 各组大鼠腹横筋膜组织氧化应激和抗氧化酶的表达水平注：每组n=6；数据均以±s表示；与Control组比较，**P<0.01，***P<0.001；与AH组比较，#P<0.05，##P<0.01；与PM组比较，*P<0.05。
图4 各组大鼠腹横筋膜组织MMPs及TIMPs的mRNA表达水平注：每组n=6；数据均以±s表示；与Control组比较，**P<0.01，***P<0.001；与AH组比较，#P<0.05，##P<0.01；与PM组比较，*P<0.05。
[1]
孙天奇, 张涛, 唐玉玲, 等. 脐疝发病机制及易感基因研究进展[J]. 生命科学, 2021, 33(10): 1260-1267.
[2]
Ding X, Zhu J, Liu A, et al. Preparation and Biocompatibility Study of Contrast-Enhanced Hernia Mesh Material[J]. Tissue Eng Regen Med, 2022, 19(4): 703-715.
[3]
买买提·依斯热依力, 阿孜古丽·阿力木江, 吾布力卡斯木·吾拉木, 等. Nox-4在慢性束缚应激致小鼠胃黏膜炎症损伤发生中的作用[J]. 中华行为医学与脑科学杂志, 2019, 28(4): 316-321.
[4]
买买提·依斯热依力, 吾布力卡斯木·吾拉木, 李义亮, 等. 心理应激诱导ROS产生在食管上皮细胞间隙增宽发生中的作用机制[J]. 医学研究杂志, 2019, 48(10): 74-79.
[5]
Durukan U, Agcaoglu O, Ozoran E, et al. The role of tissue inhibitor of metalloproteinases in the aetiology of inguinal and incisional hernias[J]. Int Wound J, 2022, 19(6): 1502-1508.
[6]
Strohalmová S, Levová K, Kuběna AA, et al. The effect of surgery on the levels of matrix metalloproteinases in patients with inguinal hernia[J]. Physiol Res, 2021, 70(4): 627-634.
[7]
Białecki JT, Myszka W, Wysocka E, et al. A comparison of the oxidative stress response in single-incision laparoscopic versus multi-trocar laparoscopic totally extraperitoneal inguinal hernia repair[J]. Wideochir Inne Tech Maloinwazyjne, 2020, 15(4): 567-573.
[8]
Pergel A, Demiral G, Tümkaya L, et al. The protective effects of topiramate on intestinal injury induced with infrarenal aortic occlusion via oxidative stress and apoptosis[J]. Clin Exp Hypertens, 2021, 43(7): 604-609.
[9]
Huang J, Yancey PG, Tao H, et al. Reactive Dicarbonyl Scavenging Effectively Reduces MPO-Mediated Oxidation of HDL and Restores PON1 Activity[J]. Nutrients, 2020, 12(7): 1937.
[10]
Madabhushi V, Tharappel J, Alluri S, et al. Hernia Repair Strength Enhanced With Antioxidants[J]. J Surg Res, 2020, 247: 144-149.
[11]
Strohalmová S, Levová K, Kuběna AA, et al. The effect of surgery on the levels of matrix metalloproteinases in patients with inguinal hernia[J]. Physiol Res, 2021, 70(4): 627-634.
[12]
Wang D, Zhang H, Li J, et al. Matrix Metalloproteinases(MMP-2) and Tissue Inhibitors of Metalloproteinases(TIMP-2) in Male Inguinal Hernia Patients at Different Ages[J]. World J Surg, 2021, 45(9): 2747-2751.
[13]
张成鹏, 徐芳, 崔逸峰, 等. 不同类型疝补片对大鼠生殖功能的影响[J/OL]. 中华疝和腹壁外科杂志(电子版), 2021, 15(1): 85-91.
[14]
于凡. 疝修补材料的研究进展及展望[J]. 外科理论与实践, 2022, 27(4): 375-379.
[15]
李宝山, 满艺, 张新, 等. 生物补片在青少年腹股沟疝经腹腹膜前修补术中的应用[J/OL]. 中华疝和腹壁外科杂志(电子版), 2023, 17(1): 20-23.
[16]
张梓榕, 陈富强, 郭迎凤, 等. 两种补片在嵌顿性腹股沟疝中的疗效[J/OL]. 中华疝和腹壁外科杂志(电子版), 2023, 17(1): 24-27.
[17]
杨建军, 李绍春, 李绍杰, 等. 生物材料补片和合成材料补片对老年腹股沟疝修补术后患者血清肿发生率的影响比较[J]. 老年医学与保健, 2022, 28(4): 887-891.
[18]
Liu J, Hou J, Liu S, et al. Graphene Oxide Functionalized Double-Layered Patch with Anti-Adhesion Ability for Abdominal Wall Defects[J]. Int J Nanomedicine, 2021, 16: 3803-3818.
[19]
李赞林, 李义亮, 克力木·阿不都热依木. 生物补片在腹腔镜下食管裂孔疝修补术中应用中的Meta分析[J/OL]. 中华胃食管反流病电子杂志, 2022, 9(1): 12-18.
[20]
刘俏男. 脂肪间充质干细胞复合生物补片治疗腹壁疝的动物实验研究[D]. 济南: 山东大学, 2022.
[1] 欧阳剑锋, 李炳权, 叶永恒, 胡少宇, 向阳. 关节镜联合富血小板血浆治疗粘连性肩周炎的疗效[J]. 中华关节外科杂志(电子版), 2023, 17(06): 765-772.
[2] 任琼, 吴东燕, 李中花, 石晶, 张静, 耿丽伟. 血清降钙素原、基质金属蛋白酶-9和可溶性细胞间黏附分子-1联合检测对绒毛膜羊膜炎的诊断价值[J]. 中华实验和临床感染病杂志(电子版), 2023, 17(03): 194-199.
[3] 曹迪, 张玉茹. 经腹腔镜生物补片修补直肠癌根治术后盆底疝1例[J]. 中华普外科手术学杂志(电子版), 2024, 18(01): 115-116.
[4] 孔博, 张璟, 吕珂. 超声技术在复杂腹壁疝诊治中的作用[J]. 中华疝和腹壁外科杂志(电子版), 2023, 17(06): 670-673.
[5] 张起尧, 刘子文. 复杂腹壁疝的解剖和生物力学基础[J]. 中华疝和腹壁外科杂志(电子版), 2023, 17(06): 674-676.
[6] 叶晋生, 路夷平, 梁燕凯, 于淼, 冀祯, 贺志坚, 张洪海, 王洁. 腹腔镜下应用生物补片修补直肠术后盆底缺损的疗效[J]. 中华疝和腹壁外科杂志(电子版), 2023, 17(06): 688-691.
[7] 柯文杰, 王勇. 成人腹壁硬纤维瘤切除术后腹壁组织修复重建的单中心临床研究经验[J]. 中华疝和腹壁外科杂志(电子版), 2023, 17(05): 535-538.
[8] 刘琪, 王殿琛, 符洋. 复杂腹壁疝诊疗问题与要点[J]. 中华疝和腹壁外科杂志(电子版), 2023, 17(05): 504-508.
[9] 刘立, 陈诚, 李新科, 刘凯, 屠昌明. 血清IL-6、hs-CRP、MMP-9联合检测在腹股沟疝无张力修补术预后评价中的价值分析[J]. 中华疝和腹壁外科杂志(电子版), 2023, 17(04): 405-409.
[10] 霍蒙蒙, 马春方, 商玉环. 延续性护理在腹壁疝日间手术中的应用[J]. 中华疝和腹壁外科杂志(电子版), 2023, 17(03): 359-361.
[11] 喻海波, 张春军. 腹腔镜腹腔内补片修补术治疗腹壁疝补片固定方法的选择与应用[J]. 中华疝和腹壁外科杂志(电子版), 2023, 17(03): 274-279.
[12] 宁鹏涛, 俞德梁, 高博欣, 徐蕾, 刘小南. 基于日间管理模式的高龄腹股沟疝Lichtenstein手术25例分析[J]. 中华疝和腹壁外科杂志(电子版), 2023, 17(02): 186-190.
[13] 李宝山, 满艺, 张新, 胡巍, 谢加东, 黄皇, 王荫龙. 生物补片在青少年腹股沟疝经腹腹膜前修补术中的应用[J]. 中华疝和腹壁外科杂志(电子版), 2023, 17(01): 20-23.
[14] 刁正文, 徐愈畅, 张杰, 张华军, 李秋霖, 陈卉. β-七叶皂苷钠联合甘油果糖治疗脑出血的临床效果分析[J]. 中华神经创伤外科电子杂志, 2023, 09(01): 32-37.
[15] 运陌, 李茂芳, 王浩, 刘东远. 微创穿刺引流联合吡拉西坦、乌拉地尔治疗基底节区高血压性脑出血的临床研究[J]. 中华脑科疾病与康复杂志(电子版), 2023, 13(05): 278-285.
阅读次数
全文


摘要

版权所有 中华医学会 中华医学电子音像出版社有限责任公司  京ICP备14006079号-1  网络出版服务许可证：(署)网出证(京)字第075号