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中华疝和腹壁外科杂志(电子版) ›› 2018, Vol. 12 ›› Issue (06) : 413 -417. doi: 10.3877/cma.j.issn.1674-392X.2018.06.004

所属专题： 文献；

论著

生长因子覆膜纳米纤维疝补片的制备及修补大鼠腹壁缺损的实验研究
陈友雷1, 贾忠2,†(), 张方捷3, 郭巨锋4   
  1. 1. 310000　浙江大学医学院附属杭州市第一人民医院急诊外科
    2. 310000　浙江大学医学院附属杭州市第一人民医院肝胆外科
    3. 310000　浙江大学医学院附属杭州市第一人民医院疝和腹壁外科
    4. 310000　浙江大学医学院附属杭州市第一人民医院乳腺外科
  • 收稿日期:2018-06-01 出版日期:2018-12-18
  • 通信作者: 贾忠
  • 基金资助:
    杭州市科技发展计划项目资助(No.20150733Q04)

Preparation of growth factor coated nanofiber hernia patch and experimental study of repairing abdominal wall defects in rats

Youlei Chen1, Zhong Jia2,†(), Fangjie Zhang3, Jufeng Guo4   

  1. 1. Department of Emergency Surgery, Zhejiang University School of Medicine, Hangzhou 310000, China
    2. Department of Hepatobiliary Surgery, Zhejiang University School of Medicine, Hangzhou 310000, China
    3. Department of Hernia & Abdominal Wall Surgery, Zhejiang University School of Medicine, Hangzhou 310000, China
    4. Department of Breast Surgery, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou 310000, China
  • Received:2018-06-01 Published:2018-12-18
  • Corresponding author: Zhong Jia
  • About author:
    Corresponding author: Jia Zhong, Email:
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陈友雷, 贾忠, 张方捷, 郭巨锋. 生长因子覆膜纳米纤维疝补片的制备及修补大鼠腹壁缺损的实验研究[J]. 中华疝和腹壁外科杂志(电子版), 2018, 12(06): 413-417.

Youlei Chen, Zhong Jia, Fangjie Zhang, Jufeng Guo. Preparation of growth factor coated nanofiber hernia patch and experimental study of repairing abdominal wall defects in rats[J]. Chinese Journal of Hernia and Abdominal Wall Surgery(Electronic Edition), 2018, 12(06): 413-417.

目的

探究生长因子覆膜纳米纤维疝补片对大鼠腹壁缺损修复的作用。

方法

利用静电纺丝技术制备生长因子VEGF165及FGF-21覆膜纳米纤维疝补片。选取30只SD大鼠，体质量100～120 g，手术造成1 cm×1 cm的全层腹壁缺损，随机分成2组，每组15只，分别采用生长因子覆膜纳米纤维疝补片（实验组）和聚丙烯疝补片（对照组）修补。术后1、2、4周分批取材（每时间点5只）对动物一般情况观察、手术部位血清肿形成情况检测、组织学观察及炎性因子表达情况比较。

结果

成功制备生长因子VEGF165及FGF-21覆膜纳米纤维疝补片；术后2组大鼠均健康成活，未发生疝瘘等不良反应，缺损得到完整修复。2组大鼠在手术部位血清肿形成方面差异无统计学意义（P>0.05）。实验组巨噬细胞及异物巨细胞计数在术后1、2周低于对照组（56.3±7.3 vs 75.2±9.6，P<0.01；37.1±6.9 vs 53.7±8.1，P<0.05），在术后4周，2组间差异无统计学意义（P>0.05）。HE染色观察表明实验组手术部位的腹壁组织的炎症反应低于对照组。实验组手术部位腹壁组织TNF-α及IL-6 mRNA表达均明显低于对照组，差异均无统计学意义（63.67±5.10 vs 194.43± 25.39，P<0.01；84.24±8.22 vs 171.41±15.86，P<0.01）。

结论

生长因子覆膜纳米纤维疝补片具有良好的生物相容性，且能降低手术部位的炎症反应，值得进一步研究。

关键词: 大鼠, 纳米纤维疝补片, 腹壁缺损, 炎症反应
Objective

To design a nanomaterial coated with growth factor to make a hernia repair patch and examine its effect on the repair of abdominal wall defect in rats.

Methods

Growth factor VEGF165 and FGF-21 were prepared by electrostatic spinning. Thirty SD rats, weighing 100 to 120 g, were randomly divided into two groups (n=15) with full-thickness abdominal wall defect caused (1 cm×1 cm) by surgery. Growth factor coated nanofiber hernia graft and polypropylene hernia graft were used for repair. General observation of the operation objects, detection of seroma formation in the surgical site, histological observation and comparison of inflammatory factors expression were made after 1, 2 and 4 weeks of operation in batches (5 samples per time point).

Results

(1) Growth factor VEGF165 and FGF-21 coated nanofibrous hernia graft were successfully made in this study. (2) All of the experimental rats were healthy and alive after surgery, no adverse reactions such as hernia fistula occurred in the two groups and the defects were completely repaired. (3) In addition, there was no significant difference in seroma formation in the surgical site between the two groups (P>0.05). (4) The number of macrophages and foreign body macrophages in the experimental group was lower than that in the control group at 1 and 2 weeks after operation (56.3±7.3 vs 75.2±9.6, P<0.01). There was no statistically significant difference between the two groups at 4 weeks after surgery. (5) The inflammatory response of abdominal wall tissue in the surgical site of the experimental group was lower than that of the control group. (6) The expression of TNF-α and IL-6 mRNA in the abdominal wall at the surgical site in the treatment group was significantly lower than that in the control group (63.67±5.10 vs 194.43±25.39, P<0.01; 84.24±8.22 vs 171.41±15.86, P<0.01).

Conclusion

The growth factor coated nanofiber hernia graft has good biocompatibility and can reduce the inflammatory response in the surgical site, worthy further study.

Key words: Rats, Nanofiber hernia patch, Abdominal wall defect, Inflammatory responsec
图1 同轴静电纺丝流程简图
图2 载生长因子的纳米纤维疝补片SEM图片
图3 对照组与实验组SD大鼠手术部位腹壁组织不同时间点的HE染色的对比（200×）
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